First, tissue sources and availability should be weighed against the experimental requirements because time of tissue harvesting and viability may be significantly impacted.
Second, the tissue anatomy needs to be understood, especially where it determines the direction of contraction and relaxation because it will then determine the correct tissue orientation within its chamber.
Third, when first developing or establishing a preparation, it is always recommended to have a series of positive and negative controls to determine how well the preparation is functioning, in part or in whole. These controls may be mechanical, pharmacological or physiological in nature.
